CD Genomics is highlighting its amplicon sequencing service for researchers seeking focused analysis of defined genomic regions. The service combines targeted PCR amplification, short- and long-read sequencing, and customizable bioinformatics to support genetic variant detection, microbial community profiling, gene-editing validation and other genomics research applications.
Amplicon sequencing is a targeted next-generation sequencing approach that uses specifically designed primers to amplify selected genomic regions before sequencing. By concentrating sequencing capacity on defined loci, the method can generate high read depth and focused data for projects that do not require whole-genome analysis.
CD Genomics provides targeted sequencing for defined genomic regions across a range of amplicon lengths and research objectives. Short- to medium-length targets may be analyzed using Illumina paired-end sequencing, while longer or full-length amplicons may be suited to PacBio HiFi sequencing. Platform selection can be guided by target size, the need for phasing, expected sequence complexity and the desired data output.
The service can support targeted variant analysis involving single-nucleotide variants, insertions and deletions, as well as validation of selected genome-editing sites. When assay design, sequencing depth and quality-control criteria are appropriate, deep sequencing may improve sensitivity for low-frequency variants. Interpretation remains dependent on primer performance, coverage uniformity, background error, sample quality and the validation requirements of each project.
Amplicon sequencing is also widely used for microbial community studies. Targeted sequencing of 16S rRNA, 18S rRNA or ITS regions can support taxonomic profiling, diversity analysis and ecological comparisons. The choice of marker region, primer set, reference database and analytical pipeline can influence the organisms detected and the resolution achieved, making study-specific design an important part of the workflow.
For immune and molecular biology research, targeted amplicon sequencing may be applied to antibody heavy- and light-chain regions, functional genes, plasmid libraries and other defined sequence elements. Long-read approaches can provide full-length sequence information and support haplotype or variant phasing when the experimental design is suitable.
The workflow may include project consultation, target definition, primer and platform planning, sample quality control, optional PCR amplification, library preparation, indexing, sequencing and data analysis. Deliverables can include FASTQ files, quality-control summaries, amplicon assembly results, variant reports, taxonomic or functional annotations, data visualizations and a comprehensive project report.
CD Genomics also provides sequencing and bioinformatics capabilities that can help research teams coordinate targeted sequencing with downstream data processing and interpretation.
About CD Genomics:
CD Genomics provides genomics sequencing, genotyping and bioinformatics services for pharmaceutical, biotechnology, academic and research customers. Its capabilities cover targeted sequencing, whole-genome and exome studies, transcriptomics, microbiome analysis, single-cell sequencing and related genomic data analysis.
Media Contact:
Contact: Dianna Gellar
Email: contact@cd-genomics.com
Phone: +1 631 259 7705
Website: cd-genomics.com
Address: Shirley, NY, USA
For Research Use Only. Not for use in clinical diagnosis, treatment or individual health assessment.